Part:BBa_K4729737

Ptau Measurement Construct Lux

Measurement construct using
BBa_K4729001
BBa_K4729206
BBa_K4729600

Figure 1: Testing different inducible promoter systems in A. rhizogenes ARqua1.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 186
  Illegal PstI site found at 1415
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1415
  Illegal NotI site found at 606
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6909
  Illegal XhoI site found at 614
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 186
  Illegal PstI site found at 1415
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 186
  Illegal PstI site found at 1415
  Illegal NgoMIV site found at 435
  Illegal NgoMIV site found at 1223
  Illegal AgeI site found at 4566
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Mostafavi, M., Lewis, J. C., Saini, T., Bustamante, J. A., Gao, I. T., Tran, T. T., King, S. N., Huang, Z., & Chen, J. C. (2014). Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression. In BMC Microbiology (Vol. 14, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1186/s12866-014-0295-2

Seiler, V. N. (2023). Improved inducible switches for the implementation of genetic tools in Sinorhizobium meliloti and Ensifer adhaerens (Master's thesis, Philipps-University Marburg)